The report displays basic information about the PanGEA-BlastN search progress. It contains basic information about the search time, the database sequences and the query sequences. It displays the number of sequences which have been mapped ambiguously and unambiguously. The report also contains all settings used for the search. The IDs of the ESTs which could not have been mapped and the IDs of the ambigous ESTs are displayed in the end of the report. The PanGEA-BlastN report may also be used to extract the sequences of the ambiguos and not mapped ESTs (see here).
PanGEA-BlastN - 26.04.2008 21:47:39
Search finished in 0,07 min
Database sequence info
Number of individual sequences 179
Cumulative length in bp 2241043
EST sequence info
Number of tags 2155
Cumulative length in bp 431963
Search results
Similarities identified for 60 tags - percent 2,78
Unambiguous similarities identified for 48 tags - percent 2,23
Ambigious similiarity found for 12 tags - percent 0,56
No similiarity found for 2095 tags - percent 97,22
Search result output
Unambiguous search results were written into file: G:\Temp\Tocheck.aln (Use this file for Gene Expression Profiling and SNP-identification)
Ambiguous search results were written into file: G:\Temp\Tocheck_ambiguous.aln (May also be use for Gene Expression Profiling - Warning: distorts gene expression profile)
PanGEAs manage pairwise sequences utility accepts multiple files at once, thus the ambiguous and unambiguous files may be analyzed concurrently
Result report was written to file: G:\Temp\TocheckReport.txt
Used search settings
Dynamic programming modified 454 Smith-Waterman-Gotoh
Used intron identification mode (concatenate partial alginments) yes
Use a maximum distance between partial alignments no
Seeding: word length 11; minimum diagonal word count 3; low complexity threshold 10
Dynamic programing; hit score 3,00; mismatch penalty 5,00; gap exist penalty 11,00; gap extent penalty 2,00; homopolymere transgression penalty 3
FILES
Database files (genes)
G:\Temp\ShortTestFiles_BlastN\D_mel_some_genes.fa
Query sequence files (tags)
G:\Temp\ShortTestFiles_BlastN\D_mel_transcripts_some_trimmed.fa
IDs of ambigious transcripts
EST a
EST b
EST c
EST d
IDs of not identified transcripts
EST e
EST f
EST g